Deadly E. coli strain may evade EPA's test

16.feb.11
Chemical & Engineering News

Since the 1980s, the Environmental Protection Agency and other regulators have tested for pathogens like E. coli O157:H7 in water by looking for fecal bacteria. These indicator microbes are harmless, easy-to-measure inhabitants of animal and human gastrointestinal tracts. But their presence in a body of water suggests that dangerous microorganisms spread through feces may also be present.
Previous research had raised questions about whether E. coli O157:H7 outlasts indicator bacteria in the environment. So Michael Jenkins and his colleagues at the U.S. Department of Agriculture's Agricultural Research Service decided to test the reliability of the EPA's method by measuring the survival rates of E. coli O157:H7 and four species of indicator bacteria. In one experiment, they injected the E. coli strain and the indicator bacteria into small, porous chambers and then suspended the chambers in test ponds in northeast Georgia. By varying the chambers' depth in the water, the scientists could monitor the microbe's survival rate under different levels of solar radiation. In another experiment, they placed inoculated pond water in bottles in an outdoor laboratory. The researchers then measured bacteria levels at regular intervals. Both experiments exposed the bacteria to predation by other microorganisms—a common fate of microbes in the environment.
They found that in both experiments, the indicator bacteria died off significantly more quickly than E. coli O157:H7 did. For example, in the outdoor lab experiments, most cells of fecal Enterococcus—an indicator species—died in less than five days. But it took between seven and 18 days for most of the E. coli O157:H7 to die. The virulent strain appeared to be more resistant than indicator bacteria to solar radiation and to predation by other microorganisms. The findings suggest that the dangerous E. coli could be present in water even when tests for fecal indicator bacteria are negative, Jenkins says. "We need to develop methods that are going to be able to quantify the pathogens themselves," he says.